Fast and versatile analytical HPLC autosampler

Three different injection modes for high-precision and fast injection

The Product is discontinued and was replaced by AAA50AA.

The Autosampler AS 6.1L can inject from up to 768 positions when equipped with microtiter plates (either high or low formats) or from up to 108 standard 1.5 ml sample vials. Sample carryover is significantly minimized thanks to a highly-effective interior and exterior needle wash procedure. This autosampler is also fast and flexible: one complete sample injection cycle takes less than one minute, including needle wash. Three different injection modes are supported; “full loop filling” (highest precision and reproducibility), “partial loop filling” (variable volumes, e.g. for dilution series) and “µl pickup” (loss-free injection of extremely small sample volumes), allowing the user to optimize sample usage. The headspace pressure function prevents bubbles from forming in the vial during sample uptake. Precolumn derivatization is supported. For high-pressure injections of up to 700 bar, the autosampler is equipped with a so-called ILD™ valve (Intermediate Loop Decompression). This valve consists of a rotor-stator combination and includes a central port for depressurizing. For high pressure applications, the sample loop is depressurized prior to receiving the sample. This way, the sample is not diluted with solvent. Because the valve is switched extremely fast, pressure spikes are reduced. Analyses are more precise and wear of the column is reduced.

Key Features

Made in Germany

Certified quality management

Customized solutions

60 years experience

KNAUER product name	Autosampler AS 6.1L

Autosampler Flow Path	Analytical
Maximum back pressure	700 bar
Vial/plate dimensions	max. plate/vial height: 47 mm (incl. septa or capmat)
Injection volume range	0.1 µl - 10 ml depending on sample loop
Sample loop	100 µl
Dispenser syringe	250 µl
Headspace pressure	built-in compressore, only for sample vials with septum
Switching time inj. valve	< 100 ms
Piercing needle precision	± 0.6 mm
Sample tray cooling	None
Vial detection	missing vial/well plate detection by sensor
Needle wash	programmable: wash between injections and wash between vials
Wetted materials	Tefzel® (ETFE), Glass, Teflon® (PTFE), Kel-F® (PCTFE), stainless steel, PEEK, Vespel

Injection modes	full loop filling, partial loop filling and microliter pickup; PASA™ (pressure-assisted sample aspiration)
Injection accuracy	0.2 µl for 250 µl injection syringe
Injection precision	RSD (Relative Standard Deviation):full loop injection: <0.3%partial loop injection at injection volume>5 µl: <0.5%microliter pickup injection at an injection volume>5 µl: <1.0%
Sample carryover	< 0,01 % under typical conditions using needle wash; < 0.005 % under special conditions with extended needle wash
Injections per vial	max. 9 injections
Injection cycle time	minimum 7 s from the same vial, 14 s from different vials;< 60 s for>100 µl sample injection in all injection modes, incl. 300 µl needle wash
Analysis time	max. 9 h, 59 min, 59 s

Inputs	2 programmable TTL inputs (next injection, freeze, stop)
Outputs	1 programmable relay output (inject marker, auxiliary, alarm)
Control	Ethernet (LAN)
Interfaces	LAN, analog

Ambient conditions	temperature range: 10–40 °C; 50–104 °F air humidity: 20 - 80%

Power supply	95–240 V AC
Dimensions	364 x 379 x 567 mm (W x H x D)
Weight	30 kg

Certified Quality

KNAUER is known for the quality of its products and has been certified several times. We produce high-tech laboratory instruments for research, routine analysis and production.
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In addition, we have extended documentation about all our products, including CE Declarations of Conformity.
See all CE Declarations of conformity

Innovation and quality - since 1962. Made in Germany.