Two Poly [(R)-3-hydroxybutyric acid] (PHB) standards (10 - and 500 kDa) were analysed in chloroform with SEC using universal calibration. For this purpose, a KNAUER system was used in combination with a linear GPC column. For the determination of the number average molecular mass (Mn) and the mass average molecular mass (Mw) as well as the poly dispersity (PD), polystyrene (PS) and polymethylmethacrylate (PMMA) calibrations were performed in chloroform. It could be shown that this system can be successfully used for the analysis of the low molecular weight standard. The molar mass of the 500 kDa standard, on the other hand, was greatly underestimated with this method, but this is probably related to the poor solubility or the sample preparation of the higher molecular weight standard.
16-point PS calibration in chloroform
12-point PMMA calibration in chloroform
Chromatogram of the 10 kDa PHB polymer. The concentration was 1 mg/ml with an injection volume of 20 μl
Chromatogram of the 500 kDa PHB polymer. The concentration was below 1 mg/ml because the polymer was not completely dissolved
| Method | HPLC |
|---|---|
| Mode | GPC |
| Substances | Poly [(R)-3-hydroxybutyric acid] |
| Key words | PHB, Poly [(R)-3-hydroxybutyric acid], GPC, SEC, polymer, biopolymers, bioplastic, biodegradable, universal calibration, PS, PMMA, green SEC, green GPC, biobased plastic, polydispersity |
| CAS number | 26063-00-3 |
| Version | Version 1/ 10/2021 |
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