FPLC Columns
High-quality to reach your purification goals
Material
Agarose or dextran
Particle Size
20–50 or
100 µm
Max. Pressure
3 bar
Modifications
His-tag, Protein A, Protein G, DEAE, CM, Q, SP
Column Brand
Sepapure®
Flexibility
Ready-to-use cartridges (1 or 5 ml) or bulk resins
Mode of Separation
Size exclusion, ion exchange or affinity chromatography
Used for all Steps
Capture, intermediate or polishing
Applications
Desalting, buffer exchange, purification of His-tagged proteins, antibody purification, separation of charged biomolecules
High-quality FPLC columns to reach your purification goals …
Sepapure® FPLC columns are a high-quality alternative to well-known FPLC columns for Size-Exclusion (SEC), Affinity (AC) and Ion-Exchange chromatography (IEX).
Our FPLC columns come as prepacked ready-to-use 1 or 5 ml cartridge and can also be purchased as bulk resin. The media are based on agarose or dextran and are available with different modifications.
With these materials, the Sepapure® FPLC columns are the best matching biochromatography columns to your AZURA FPLC system, giving you the complete and reliable KNAUER FPLC package. Made in Germany
Please let us know how we can help.
What is your application challenge?
KNAUER Sepapure® FPLC columns for
Purification of Antibodies
For the purification of antibodies or antibody fragments, Sepapure® AC media based on agarose are available functionalized with Protein A or Protein G.
Immobilized Metall Ion Affinity
For the purification of biomolecules through interaction with a recombinant His-tag, KNAUER offers Sepapure® media based on agarose for purifying histidine- tagged proteins.
Protein Purification by Charge
Ion-exchange (IEX) chromatography can separate molecules or groups of molecules according to their charge at a specific pH. Separation is based on the reversible interaction between a charged molecule and the oppositely charged chromatography medium.
Sepapure® media based on agarose are available in weak and strong cation and anion exchange modifications.
Name | Used for.... |
Sepapure DEAE FF6 | Weak Anion Exchange |
Sepapure Q FF6 | Strong Anion Exchange |
Sepapure CM FF6 | Weak Cation Exchange |
Sepapure SP FF6 | Strong Cation Exchange |
Desalting or Buffer Exchange
Size exclusion chromatography (SEC) separates molecules on the basis of size differences. KNAUER offers desalting columns based on dextran for group separations of large molecular weight biomolecules from small molecular weight compounds and salts.
