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When they go LOW, we go HIGH - Comparing sensitivity levels for the analysis of fluorescence-labeled proteins

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Application No.: VTN0005

When they go LOW, we go HIGH - Comparing sensitivity levels for the analysis of fluorescence-labeled proteins

The use of a fluorescence detector increases the sensitivity of the chromatographic analysis. In this Tech Note we evaluated the different sensitivity modes of the fluorescence detector with a fluorescein-labeled protein and compared it to the UV signal.

Technical Data

Application

Method FPLC

Separation conditions

Mode SEC
Substances Fluorescein isothiocyanate isomer I (FITC), Bovine Serum Albumin (BSA)
Key words SEC, FPLC, AZURA FPLC, fluorescence, BSA, FITC, fluorescein-labeled protein
CAS number 3326-32-7, 9048-46-8

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