The extended dynamic range (EDR) option for the AZURA RID 2.1L refractive index detector was investigated with a simple sample consisting of two common sugars. Calibration curves covering the range 700 μRIU to 2300 μRIU were generated with activated and deactivated EDR. A gain of about 65 % in dynamic range could be demonstrated over this range.
Overlay of an injection with 50 μL, blue=without extension, red=with extension (+100 %)
Linearity of glucose calibration with (red, R=0.9924) and without (blue, R=0.8087) EDR option
| Method | HPLC |
|---|
| Mode | RP |
|---|---|
| Substances | Glucose, saccharose |
| Key words | Sugar, glucose, saccharose, scale up, RID 2.1L, extended range, extended dynamic range, semi-preparative, preparative |
| CAS number | 58367-01-4, 57-50-1 |
Analytical HPLC system, ideally suited for the determination of sugars, organic acids and alcohols.
Article No.: 981701200
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AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 700 bar
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Article No.: 25WE184E2J
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