The separation of compounds chosen among purine and pyrimidine bases was studied by using hydrophilic interaction chromatography (HILIC). The retention of the analytes was studied by varying ACN%, ammonium formate concentration, pH, and column temperature. The results obtained confirmed the elution order of nucleobases and nucleosides on their hydrophobicity.
3,5- Di-tert-butyltoluene (9.2 mg/ml) dead time marker, Adenine (0.15 mg/ml), Cytosine (0.20 mg/ml), Uracil (0.14 mg/ml)
| Method | HPLC |
|---|
| Mode | HILIC |
|---|---|
| Substances | 3,5- Di-tert-butyltoluene, Adenine, Cytosine, Uracil |
| Key words | HILIC, AZURA Compact, Eurospher II HILIC, Purine, Pyrimidine |
| CAS number | 15181-11-1, 66-22-8, 73-24-5, 71-30-7 |
Article No.: 15CE120E2J
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