Ion exchange chromatography is one of the most widely used FPLC techniques for protein separation and purification. Depending on the charge of the sample and the resin cation or anion exchange chromatography is used. This
application describes an easy separation of model proteins and explains how cation exchange chromatography works.
Chromatograms of the separation of Ribonuclease A (1), Cytochrome C (2), and Lysozyme (3)
Principle of cation exchange chromatography
|Substances||Cytochrome C, Lysozyme, Ribonuclease A|
|Key words||FPLC, IEC, CIEC, AZURA FPLC, Sepapure SP, Sepapure CM|
|CAS number||9007-43-6,12650-88-3, 9001-99-4|
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