The quantification of oligonucleotides can easily be done by UV detection because oligonucleotides show strong absorption at 260 nm. Expanding the UV method for quality control with mass spectrometry results in mass confirmation and impurity identification.
Q1 spectrum of oligonucleotide sample from 10.95 to 11.27 minutes, m/z 500 – 1250 Da
Q1 spectrum in a range from 610 – 650 Da, m/z=13, main peak (642.94) and N-1 impurity (618.21)
XIC of SIM at m/z=13 (left), enhanced view of XIC (right), blue – Main peak, red – NPO, green – NDEP
Area % of impurities from SIM at m/z=13 in relation to main peak (100 %)
| Method | LC-MS |
|---|---|
| Mode | RP |
| Substances | Oligonucleotide |
| Key words | HPLC, oligonucleotide, ion pairing, LC-MS, quality control, active pharmaceutical ingredient, API |
| CAS number | n/a |
| Version | Version 1 04/2024 |
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