The purification of compounds from natural sources can be achieved by various techniques, including single- and multi-column liquid chromatography. However, the development of efficient, fast and cost-effective methods for the purification of natural products from i.e. extracts can be a difficult and challenging task due to the complexity of the matrix. In contrast to conventional purification methods such as distillation, preparative chromatography uses less energy, consumes less water and chemicals while achieving higher yields [1]. In this application, a batch process for the purification of CBD (cannabidiol) from a CBD-rich cannabis extract was established. The application is devided into two Application Notes, first, an analytical method was developed and optimized. Second, CBD was purified using the scaled-up method on a preparative system.
System setup of analytical HPLC system
Chromatograms of the overview gradient with EtOH as eluent B
Chromatogram of the focus gradient with EtOH as eluent B
Overlay chromatograms in analytical overload tests
System setup of preparative HPLC system
Scale up. Sample: CBD-rich extract sample
| Method | Preparative HPLC |
|---|---|
| Mode | RP |
| Substances | Cannabidiol (CBD), Cannabichromene (CBC), Tetrahydrocannabinol (THC) |
| Key words | HPLC, preparative chromatography, RP, purification of natural products, method development, scale up, CBD, CBC, THC |
| CAS number | 13956-29-1, 20675-51-8, 1972-08-3 |
| Version | Version 01/11.07.2024 |
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