Since the change in German narcotics law in 2017 (§1 Abs. 1, Betäubungsmittelgesetz; BtMG) the need for reliable and robust HPLC methods for quality control has drastically increased. Six common cannabinoids of high medicinal interest Cannabidiol (CBD), Cannabidiolic acid (CBDA), Cannabinol (CBN), Δ8-Tetrahydrocannabinol (Δ8-THC), Δ9-Tetrahydrocannabinol (Δ9-THC) and Δ9-Tetrahydrocannabinolic acid (Δ9-THCA) were quantified on the KNAUER AZURA® HPLC plus system according to the monograph of German Pharmacopeia 2018 (Deutsches Arzneibuch; DAB). The method was verified using an authorized medicinal cannabis flower available on the market. The assignment of the analytes was made with chemical reference standards.
Chromatogram of a 10 μg/mL standard mix measured with the DAB method; R=1.3 between Δ8-THC and Δ9-THC; blue - 225 nm, red – 306 nm, 1 - CBDA, 2 - CBD, 3 - CBN, 4 - Δ9-THC, 5 - Δ8-THC, 6 - Δ9-THCA.
| Method | HPLC |
|---|---|
| Mode | RP |
| Substances | CBD, CBDA, CBN, Δ9-THC, Δ8-THC, Δ9-THCA |
| Key words | bediol, DAB, CBD, CBDA CBN, Δ9-THC, Δ8-THC, Δ9THCA, cannabis, german pharmacopeia, cannabinoids, cannabis flowers, cannabis sativa L. |
| CAS number | 13956-29-1, 1244-58-2, 521-35-7, 5957-75-5, 1972-08-3, 23978-85-0 |
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