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Eurospher II Chiral
The chiral stationary phases (CSPs) of KNAUER’s Eurospher® II Chiral HPLC columns were designed to tackle a wide range of chiral separation tasks.
The chiral stationary phases (CSPs) of KNAUER’s Eurospher® II Chiral HPLC columns were designed to tackle a wide range of chiral separation tasks.
Depending on the analytes, three different chiral selectors are available:
| Chiral column | HPLC mode | USP code | Description | Chiral selector |
|---|---|---|---|---|
| Chiral AM | NP | L51 | polysaccharide chiral stationary phase | Amylose-tris-(3,5-dimethylphenylcarbamate) |
| Chiral AM-R | RP | L51 | polysaccharide chiral stationary phase | Amylose-tris-(3,5-dimethylphenylcarbamate) |
| Chiral OM | NP | L40 | polysaccharide chiral stationary phase | Cellulose-tris-(3,5-dimethylphenylcarbamate) |
| Chiral OM-R | RP | L93 | polysaccharide chiral stationary phase | Cellulose-tris-(3,5-dimethylphenylcarbamate) |
| Chiral NR | NP | n/a | immobilized brush-type phase | p-electron donor- and p-electron acceptor groups |
| Chiral NR-R | RP | n/a | immobilized brush-type phase | p-electron donor- and p-electron acceptor groups |
The following table gives an overview of the differences between the three different Eurospher II Chiral phases.
| Eurospher II Chiral NR | Eurospher II Chiral OM | Eurospher II Chiral AM |
|---|---|---|
| Analytes need an oxygen at or near the chiral center and additionally an aromatic ring | Highly versatile chiral phase | Highest generality of all Eurospher II Chiral phases |
| Elution more predictable than with Chiral AM and OM phases | Analyte does not need to carry an aromatic ring | Analyte does not need to carry an aromatic ring |
| Inversion of elution order is possible | No inversion of elution order possible | No inversion of elution order possible |
| Very high capacity and thus ideally suited for preparative HPLC | Scale up in the preparative HPLC range is possible | Scale up in the preparative HPLC range is possible |
| Stable in all common HPLC eluents | Caution! Not stable in all HPLC eluents! | Caution! Not stable in all HPLC eluents! |
Eurospher II Chiral AM is equivalent to Daicel's well-known Chiralpak AD*, and Eurospher II Chiral OM is equivalent to Chiralcel OD* phases. KNAUER offers these chiral stationary phases for two different separation modes: Eurospher II Chiral AM and Chiral OM are designed for separations in normal phase (NP) mode, Eurospher II Chiral AM-R and Chiral-OM-R for separations in reversed phase mode.
Both Eurospher II Chiral AM and Chiral OM columns are able to separate neutral, acidic and basic racemates.
*) Chiralpak AD and Chiralcel OD are brand names of Daicel Corporation.
|
Silica gel |
ultra pure, > 99.99 % |
|
Metal content |
< 10 ppm |
|
Particle size |
3 μm, 5 μm, 10 μm |
|
Particle form |
spherical |
|
Pore size of base material |
1000 Å |
|
Chiral selector |
amylose-tris-(3,5-dimethylphenylcarbamate) for Chiral AM cellulose-tris-(3,5-dimethylphenylcarbamate) for Chiral OM |
Eurospher II Chiral NR is an immobilized brush-type phase with very broad generality and complementary selectivity to Eurospher II Chiral AM and OM. In many cases it is similar to Eurospher II Chiral AM or Chiral OM, but much more stable. The Chiral NR selector is covalently bound, so that you can use all HPLC eluents.
Due to its high loadability it is perfectly suited for preparative separations. This CSP is offered for normal phase mode (Eurospher II Chiral NR) and for reversed-phase mode (Eurospher II Chiral NR-R).
Eurospher II Chiral NR is similar to Daicel's immobilized Chiralpak IA, IB and IC* columns.
*) Chiralpak IA, IB and IC are brand names of Daicel Corporation
|
Silica gel |
ultra pure, > 99.99 % |
|
Metal content |
< 10 ppm |
|
Particle size |
Eurospher II Chiral NR: 3 μm, 5 μm, 8 µm, 12 µm Eurospher II Chiral NR-R: 8 µm, 12 µm |
|
Particle form |
spherical |
|
Pore size of base material |
100 Å |
KNAUER offers a chiral screening service for your samples. We will find the best suited column for you!
For more information, click below.
