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Analytical wide pore HPLC Columns for the determination of biomolecules bigger than 5000 Da (USP L7)
For middle sized proteins up to about 20,000 Da, C8 phases with shorter carbon chains compared to C18 phases are recommended.
For middle sized proteins up to about 20,000 Da, C8 phases with shorter carbon chains compared to C18 phases are recommended.
Ultra pure, spherical wide pore HPLC phase based on wide pore silica gel
Unpolar, monomeric C8 modification, endcapped
4.5 % carbon content (conventional endcapping)
Properties:
Separation mechanism: Hydrophobic interaction
Key features:
Eurosil Bioselect 300 C8 has been specifically developed for the determination and purification of peptides, proteins, and oligonucleotides up to MW approx. 20,000 Da. The optimized manufacturing process guarantees excellent batch–to-batch reproducibility. The Eurosil Bioselect sorbent is the first choice for Biochromatography with silica material.
Technical data:
C8 phase based on ultra-pure silica gel (purity > 99 %), metal content < 10 ppm, pH-stability 2 - 8, pore size 300 A, particle size 3 μm, 5 μm, 10 μm (15 μm, 20 – 45 μm on request)
Recommended application areas:
hydrophilic biomolecules, peptides, peptide mapping after enzymatic digestion
Spherical high performance HPLC phase based on silica gel, Monomeric C8 (Octyl) modification, full hydrophobic endcapping with 7 % carbon content.
ProntoSIL C8 SH is a classical C8-type stationary phase. It is fully endcapped. Due to the bonding technology it shows an excellent shape selectivity and stability even at pH 1. The C8 SH-bonding type is available in several pore and particle sizes. The 300 Å packings show excellent properties for the separation of large biomolecules like proteins and peptides. The 5 μm support is also available in 60 Å.
