How to write methods in PurityChrom MCC Plus - a short checklist

1. Methods are called “Time Control Files”.

2. You can write your method time-, volume-, or column volume-based. However, please note that PurityChrom MCC Plus calculates the volume and the column volume based on the flow rates summed up from all pumps in the system.

3. You can stop your pumps if you pause your run with the “Hold” button. Therefore check “Stop Pumps at Time Control Hold” in “Options”.

4. Start your method at time point 0 with the function “Flowrate” for each pump of the system. You can choose a flow rate of 0 ml/min for pumps you want to start later.

5. Choose the start position of all your used valves at time point 0.

6. Choose your wavelength (function “Wavelength”) and your required channels with the function “Start Chromatogram” from time point 0.02 (not 0.0).

7. Don’t forget to program an autozero for your UV signal.

8. To start fractionation using a fraction collector, change the position of the valve of your fraction collector from “Waste” to “Fraction”. The function “Collector” enables you to move the collector arm to the wished position (“Step”: The arm will perform a step to the next position). Don’t program the function “Collector” at time point 0. To set a maximum volume for fractions use the function “Fraction Limit”. The current position of the fraction collector arm will be remembered until the program is switched off and on again.

9. To start fractionation using a fraction valve, change the position of the valve. To set a volume use the function “Fraction Limit”. The current position of the fraction valve will not be remembered by the software.

10. Program the function “Stop All” at the end of your method. The function “Stop Chromatogram” is only needed if you want to restart your method automatically (function “Restart Time Control File”), if you want to link another method to your method (function “Load new File”).