Peptides, crucial in therapeutic advancements, are synthesized through refined methods in biotechnology. High-performance liquid chromatography (HPLC) plays a pivotal role, ensuring the purity of synthesized peptides. Linear scale-up of HPLC methods becomes imperative for enhancing productivity and method robustness in pharmaceutical and analytical laboratories. This process involves meticulous adjustments to parameters, such as column size and solvent composition, to maintain consistent chromatographic performance. Scaling up begins with optimizing the method on a larger scale, ensuring minimal sample loss while maximizing productivity.
Analytical overview gradient; (1) Angiotensin I
Focused gradient and wash step
Top to bottom: 100, 50, 25, 10, 5 µl injection volume
Preparative run (zoomed); 2000 µl injection volume; 2.5 ml fractions
| Method | Preparative HPLC |
|---|---|
| Mode | RP |
| Substances | Angiotensin I |
| Key words | HPLC, preparative, RP, purification, Angiotensin, Angiotensin I, TFA, Trifluoroacetic acid, scale-up, overload, UV, hormone, Peptides, Biotechnology, High-performance liquid chromatography ( |
| CAS number | 484-42-4; 9041-90-1 |
| Version | Version 1 / 07/2024 |
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