Using valves in FPLC is most often necessary due to manual injection, column switching or fractionation. But also common analytical HPLC applications can be improved by adding valves. Widespread analytical application areas are for example column screenings to find the suitable column. A large variety of valves is available, but not all are suitable for the screening of pressure sensitive columns. This note describes an application of a KNAUER 8 port 2-position valve in a screening process with Eurokat columns.
Switching of 8 port 2-position valve. Position 1 – left, position 2 - right
Mixed standard on Eurokat Na. 1 - Cellobiose, 2 - Mannitol/Glucose, 3 - Xylose, 4 - Xylitol, 5 - Glycerin/Arabinose
Mixed standard on Eurokat Ca. 1 - Cellobiose, 2 - Glucose, 3 - Xylose, 4 - Arabinose, 5 - Glycerin, 6 - Mannitol, 7 - Xylitol
| Method | HPLC |
|---|---|
| Mode | Ion exclusion, Ligand exchange |
| Substances | Cellobiose, mannitol, glucose, xylose, xylitol, glycerin, arabinose |
| Key words | Eurokat, carbohydrates, column screening, sugar, PSDVB, polymer column, degree of polymerization, RID, refractive index |
| CAS number | 528-50-7, 87-78-5, 58367-01-4, 609-06-3, 87-99-0 , 56-81-5 , 147-81-9 |
| Version | 1/10-2020 |
Technical note, VTN0008
AZURA® Pump P 6.1L (HPG), with 10 ml pump head (stainless steel), degasser and mixer (400 µl)
Article No.: APH35EA
Show product
AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 700 bar
Article No.: AAA00AA
Show product
AZURA® RID 2.1L analytical refractive index detector with flow cell
Article No.: ADD31
Show product
Column thermostat AZURA CT 2.1 for constant temperatures and reproducible results
Article No.: ATC00
Show product
VU 4.1 valve drive for V 4.1 valves
Article No.: AWA01
Show product
2-position valve, 8 Port, DLC stainless steel, Vespel®, 1/16", 1200 bar, 0.3 mm bore size
Article No.: AVC38AC
Show product
Article No.: 30GX360EKN
Show product
Article No.: 30GX210EKN
Show product
ClarityChrom® single instrument license for one time base
Article No.: A1670
Show productWe are happy to help you
