Ion exchange chromatography is one of the most widely used FPLC techniques for protein separation and purification. Depending on the charge of the sample and the resin cation or anion exchange chromatography is used. This application describes an easy separation of model proteins and explains how anion exchange chromatography works.
Chromatograms of the separation of Conalbumin (1), α-Lactoglobin (2) and soy bean Trypsin inhibitor (3)
Overlay of chromatograms on weak (A) and strong (B) anion exchange chromatography columns.
Principle of anion exchange chromatography
| Method | FPLC |
|---|
| Mode | IEC |
|---|---|
| Substances | Conalbumin, a-Lactalbumin, soy bean Trypsin inhibitor |
| Key words | FPLC, IEC, AIEC, AZURA FPLC, Sepapure Q, Sepapure DEAE |
| CAS number | 1391-06-6, 9051-29-0, 9035-81-8, |
for up to 10 mL/min
Article No.: SYS872112122
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for up to 50 mL/min
Article No.: SYS862113122
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