Vitamins can be divided into fat-soluble and water-soluble vitamins. Water-soluble vitamins dissolve in water, which means these vitamins and nutrients dissolve quickly in the body. Examples for water soluble vitamins are vitamin C and the vitamin B complex: thiamin (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), vitamin B6,biotin (B7), folic acid (B9), cyanocobalamin (vitamin B12). In this work, eight water-soluble vitamins were separated and quantified in less than 10 min.
Absorption spectrum of cyanocobalamin
Chromatogram of standard mix, 1 ascorbic acid, 2 nicotinic acid, 3 thiamine, 4 pyridoxine, 5 nicotinamide, 6 folic acid, 7 cyanocobalamin, 8 riboflavin
| Method | HPLC |
|---|
| Mode | RP |
|---|---|
| Substances | ascorbic acid, nicotinic acid, thiamine, pyridoxine, nicotinamide, cyanocobalamin, riboflavin |
| Key words | ascorbic acid, nicotinic acid, thiamine, pyridoxine, nicotinamide, cyanocobalamin, riboflavin, water-soluble vitamins, dietary supplement |
| CAS number | 50-81-7, 59-67-6, 67-03-8, 65-23-6, 98-92-0, 68-19-9, 83-88-5 |
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