Pressure fluctuations caused by pump pulsation can present a challenge in FPLC, especially when working with the pressure sensitive columns used in size exclusion chromatography applications. Here we analyzed the performance of the KNAUER PEEK pulse damper in combination with the compact pump AZURA P 4.1S. The pulse damper was able to reduce pressure pulsation by up to 92%. The best working conditions for the pulse damper were determined at small flow rates in combination with a low back pressure within the system. Reducing the degree of pressure pulsation prevents potential damage to the column packing and therefore it is highly recommended to include the pulse damper within the flow path of the AZURA SEC FPLC system. Here, two system configurations are reviewed in detail.
Flow scheme of AZURA SEC FPLC system with pulse damper DOWNSTREAM of the pressure sensor
System set up of AZURA SEC FPLC system with pulse damper installed DOWNSTREAM of the pressure sensor
Flow scheme of AZURA SEC FPLC system with pulse damper and additional back pressure regulator installed UPSTREAM of the pressure sensor
System set up of AZURA SEC FPLC system with pulse damper and additional back pressure regulator installed UPSTREAM of the pressure sensor
Overlay of the pressure signal at different flow rates, approx. 1.5 bar back pressure and water as eluent. Pressure signal without pulse damper (red), with pulse damper (blue); 0.1 ml/min
Overlay of the pressure signal at different flow rates, approx. 1.5 bar back pressure and water as eluent. Pressure signal without pulse damper (red), with pulse damper (blue); (B) 0.5 ml/min
Overlay of the pressure signal at different flow rates, approx. 1.5 bar back pressure and water as eluent. Pressure signal without pulse damper (red), with pulse damper (blue); 1.0 ml/min
Comparison of the percent reduction of the pulsation/amplitude for different flow rates at different back pressures: (dark blue) ~1.5 bar; (red) ~4.5 bar; light blue ~10 bar with water
| Method | FPLC |
|---|---|
| Mode | SEC |
| Substances | - |
| Key words | FPLC, SEC, pulse damper, AZURA SEC FPLC, biocompatible |
| CAS number | - |
| Version | Version 1 / 10/2021 |
Pressure fluctuations caused by pump pulsation can present a challenge in FPLC, especially when working with the pressure sensitive columns used in size exclusion chromatography applications. Here we analyzed the performance of the KNAUER PEEK pulse damper in combination with the compact pump AZURA P 4.1S. The pulse damper was able to reduce pressure pulsation by up to 92 %. The best working conditions for the pulse damper were determined at small flow rates in combination with a low back pressure within the system. Reducing the degree of pressure pulsation prevents potential damage to the column packing and therefore it is highly recommended to include the pulse damper within the flow path of the AZURA SEC FPLC system. Here, two system configurations are reviewed in detail.
for up to 10 mL/min
Article No.: SYS872101122
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This pulse damper combines high damping performance with reliable, membrane-free assembly. Fully biocompatible, it can be easily integrated into all AZURA FPLC systems.
Article No.: AZZ10NB
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