One of the 10 most dangerous chemicals in the world is aflatoxin B1. For both, humans and animals the consumption of the toxic aflatoxins can lead to serious health damage. They are produced by mold fungi on food and feed products. In this AppNote, a simple, robust and highly sensitive method for the analysis of aflatoxins in food and feed is provided to ensure consumer safety.
Chemical structures of the four aflatoxins and the reaction mechanism of the photochemical activation
Chromatogram of a mixture including equal parts of extraction products from peanuts, pistachios, cereal puree and rusk for babies and dried fruits
Concentration curves for the four aflatoxins with correlation coefficient. The empiric determined LOD for the aflatoxins B1/G1 was 0.05 ng/mL and for B2/G2 0.015 ng/mL
Aflatoxin contaminations from moldy food can be a big health risk
Method | HPLC |
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Sample | Aflatoxin B1, B2, G1 and G2 |
Separation column | Eurospher II C18 100-3 150-4.6 mm |
Stationary phase | RP |
Mode | RP |
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