Extraction of natural components from plants that are used for medical treatments is widely applied. Often the extraction process results in an oil, such as the chamomile blue oil with many different compounds. Usually only some of these components are responsible for the medical properties of the whole extract. In chamomile oil, chamazulene is one of these medical active compounds. Here, the preparative purification of chamazulene by HPLC from chamomile blue oil is described.
VPH0071 Chromatogram chamazulene purification. Collected fraction highlighted in grey. 2 mL injection volume; x – chamazulene peak; C18 20 x 250 mm; 10 μm; 25 mL/min.
VPH0071 Overlay fraction from Fig. 4 in blue and chamazulene standard (0.75 mg/mL) in red on analytical column
| Method | Preparative HPLC |
|---|---|
| Mode | RP |
| Substances | Chamazulene |
| Key words | Chamazulene, scale-up, preparative LC, RP, pharma, purification |
| CAS number | 529-05-5 |
Purification of chamazulene by preparative HPLC and its scale-up
AZURA® Pump P 2.1L with 100 ml pump head (stainless steel)
Article No.: APE20KA
Show product
LPG module for Pump P 2.1L ternary up to 220 ml/min (stainless steel)
Article No.: AZZ00AB
Show product
Pump head 250 ml, stainless steel 200 bar
Article No.: A4021-1
Show product
AZURA® Detector UVD 2.1L with deuterium lamp without flow cell, incl. test cell
Article No.: ADA01XA
Show product
0.5 mm path length, 3 µl, 1/16", 200 bar, stainless steel, classical KNAUER flow cell
Article No.: A4069
Show product
Preparative Column
Article No.: 15OE181E2N
Show product
Basic License for one system
Article No.: A2650
Show productWe are happy to help you
