When using trifluoroacetic acid (TFA) as eluent modifier in liquid chromatography its self-absorption properties can present challenges for baseline stability. As consequence the pumps pulsation, normally less noticeable can become indirectly visible in detector baseline. This can lead to a negative effect on limit of detection (LOD) and limit of quantification (LOQ) for chromatographic methods. Mitigation of this phenomenon can be easily achieved by adjusting the pump mixer volume appropriately.
Left – Free silanol groups (blue), able to deprotonate; Right – silanol groups cannot deprotonate since TFA deprotonated first (red)
Staggered overlay of gradients for P 6.1L pump with 100 µl (black), 200 µl (red), 400 µl (blue), 600 µl (green) mixer
Zoom on baseline for P 6.1L pump with 100 µl (black), 200 µl (red), 400 µl (blue), 600 µl (green) mixer
Effect on gradient delay volume for P 6.1L pump with 100 µl (black), 200 µl (red), 400 µl (blue), 600 µl (green) mixer
| Method | HPLC |
|---|---|
| Mode | RP |
| Substances | Trifluoroacetic acid, TFA |
| Key words | HPLC, RP, TFA, pulsation, baseline, noise, modifier, mixing, mixer, ripple |
| CAS number | 76-05-1 |
| Version | version 1 / 03/2024 |
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