Steviol glycosides are the main sweetening compounds in Stevia rebaudiana and can be used as natural sugar substitutes. This method provides a fast determination of six steviol glycosides using the AZURA® HPLC Plus System. With a fast separation under 10 min and an optimized gradient, the developed method is suitable for a fast quality control of stevia products. Furthermore, the robustness for this method was validated using DryLab simulation software.
Chromatogram of calibration standard at a concentration of 0.1 mg/m; 1) rebaudioside A, 2) stevioside, 3) rebaudioside C, 4) dulcoside A, 5) rebaudioside B, 6) steviolbioside
Chromatogram of dried Stevia leaves sample after SPE purification; 1) rebaudioside A, 2) stevioside, 3) rebaudioside C, 4) dulcoside A, 5) rebaudioside B, 6) steviolbioside
Chromatogram of Stevia sweetener sample; 1) rebaudioside A, 2) stevioside, 3) rebaudoside C, 4) dulcoside A, 5) rebaudioside B, 6) steviolbioside
| Method | HPLC |
|---|---|
| Mode | RP |
| Substances | Rebaudioside A, stevioside, rebaudioside B, rebaudioside C, dulcoside a, steviolbioside |
| Key words | Stevia rebaudiana, steviol glycosides, rebaudioside A, stevioside, rebaudioside C, dulcoside A, rebaudioside B, steviolbioside, stevia sweetener, Stevia, SPE, sugar substitute |
| CAS number | 58543-16-1, 57817-89-7, 63550-99-2, 64432-06-0, 58543-17-2, 41093-60-1 |
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