The quality control of hemp and cannabis before human consumption e.g., the determination of pesticide residues and mycotoxins in cannabis biomass and its derived products, is mandatory. The U.S. Food & Drug Administration (FDA) and Commission Regulation (EC) No 2023/915 set limit values for mycotoxins in human food and animal feed. Organizations like AOAC are developing method requirements for mycotoxin determination in cannabis matrices. AOAC SMPR®2021.010 defines aflatoxins B1/B2, aflatoxins G1/G2 and ochratoxin A as analytes of interest and specifies limits of quantification and qualification for cannabis biomass and cannabis derived products. Here, we describe sample preparation methods and an analytical chromatography LC-MS/MS method to detect mycotoxins in different hemp matrixes at ppb level.
Simplified overview of sample preparation procedures
Overlay of XIC from quantifier transitions, mycotoxin mix standard at L2
Recovery [%] of mycotoxins for all samples with sample preparation P4
TIC of spiked hemp seed sample with different sample preparations; P1 – blue, P4 – red; right corner: XIC of P4 for hemp seeds
| Method | LC-MS |
|---|---|
| Mode | RP |
| Substances | aflatoxin G2, aflatoxin G1, aflatoxin B2, aflatoxin B1, ochratoxin A, zearalenone |
| Key words | mycotoxins, aflatoxins, ochratoxin A, zearalenone, cannabis, hemp, fluorescence, MS, sample preparation, QuEChERS, immunoaffinity chromatography, IAC, solid phase extraction, SPE, LC-MS/MS |
| CAS number | 7241-98-7, 1402-68-2, 7220-81-7, 1162-65-8, 303-47-9, 17924-92-4 |
| Version | version 1 07/2024 |
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