Although time is relative, the total run time of a method is an important factor when it comes to high throughput analysis. Saving time means saving money. With the exemplary separation of compounds that are present in anti-epileptic drugs, the KNAUER AZURA UHPLC system equipped with the new binary AZURA P 8.1L pump, thanks to the low dispersion and operating pressure up to 1240 bar, allows to speed up your method and increase throughput preserving resolution.
Chromatogram at 0.2 ml/min, (1) caffeine, (2) PEMA, (3) ethosuximide, (4) primidone, (5) carbamazepine, (6) phenytoin
Chromatogram at 1.5 ml/min, (1) caffeine, (2) PEMA, (3) ethosuximide, (4) primidone, (5) carbamazepine, (6) phenytoin
Overlay of chromatograms at different flow rates, dark blue- 0.2 ml/ min, red – 0.6 ml/min, black – 1.2 ml/min, light blue – 1.5 ml/min
Standard deviation of retention time over 10 replicates for each flow rate
Normalized overlay of primidone (peak 4), dark blue- 0.2 ml/min, red – 0.6 ml/min, black – 1.2 ml/min, light blue – 1.5 ml/min
Van Deemter plots
| Method | UHPLC |
|---|---|
| Mode | RP |
| Substances | Caffeine, ethosuximide, primidone, carbamazepine, phenytoin, 2-ethyl-2-phenylmalonamide monohydrate |
| Key words | (U)HPLC, RP, AZURA, P 8.1L, anti-epileptic drugs, high throughput, analysis time, band broadening, extra column volume, symmetry, van Deemter, system volumes |
| CAS number | 80866-90-6, 58-08-2, 77-67-8, 125-33-7, 298-46-4, 57-41-0 |
| Version | version 1 06/2023 |
AZURA® P 8.1L UHPLC pump with a maximum delivery pressure of up to 124 MPa/1240 bar for ultra-fast and high-resolution applications
Article No.: APF45PA
Show product
AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 1240 bar, with sample cooling/heating
Article No.: AAA11AA
Show product
AZURA® Detector DAD 6.1L Diode array detector DAD 6.1L without flow cell 190 - 1000 nm, incl. test cell
Article No.: ADC11
Show product
10 mm path length, 2µl, 1/16", 50 bar, LightGuide Flow cell cartridge for AZURA® Detector DAD/MWD
Article No.: AMC19XA
Show product
Column thermostat AZURA CT 2.1 for constant temperatures and reproducible results
Article No.: ATC00
Show product
ClarityChrom® single instrument license for one time base
Article No.: A1670
Show product
ClarityChrom® option for PDA data processing
Article No.: A1676
Show product
Stainless steel, 0.1 mm ID, 700 mm length, red
Article No.: AZF44
Show product
UHPLC 1240 bar system with a binary HPG pump, featuring adaptive pulsation compensation for ultra-fast and high-resolution liquid chromatography applications.
Article No.: 908603200
Show productWe are happy to help you
