Specific regeneration methods must be used for different types of polymer columns. Appropriate regeneration methods for specificpolymer based columnsare described below. This is our recommended regeneration procedure of polymer columns:
- Eurokat H, CarbEx H-Form, Hamilton HC-75 Hydrogen Form
Rinse the column for 4 to 6 hours with 0.05 N sulphuric acid at 60 °C, using aflow rate of 0.2 ml/min - Eurokat Ca, CarbEx Ca-Form, Hamilton HC-75 Calcium Form,Hamilton HC-40 Calcium Form
Rinse the column for 4 to 6 hours with a 0.25 M calcium nitrate solution at 60 °C, using a flow rate of 0.2 ml/min - Eurokat Pb, CarbEx Pb-Form, Hamilton HC-75 Lead Form
Rinse the column for 4 to 6 hours with a 0.25 M lead nitrate solution at 60 °C, using a flow rate of 0.2 ml/min - Hamilton PRP-1, PRP-3, PRP-Infinity
Rinse the column with 20 column volumes of water
Run a linear gradient from 100% water to 100% acetonitrile
Repeat the procedure 3 times - Hamilton PRP X-100
Rinse the column with 20column volumes of water
Rinse the column with 20 column volumes of methanol with 1% 6 N nitric acid - Hamilton PRP X-200, PRP X-300
Rinse the column with 20 column volumes of water
Inject 100 μl of 1 N nitric acid, 5 times consecutively - Hamilton RCX-10
Rinse the column with 20 column volumes of a 0.1 N sodium hydroxide solution - Hamilton RCX-30
Rinse the column with 60 column volumes of a 0.1 N sodium hydroxide solution