Size exclusion chromatography (SEC) is a popular FPLC techniques used for protein purification. Molecules are separated according to their size. Depending on the aim of the purification high resolution fractionation or group separation is used. This application describes group separation with Sepapure Desalting and shows examples for the separation of proteins from dyes and for protein desalting.
Separation of BSA and 5-FAM
Desalting of BSA
| Method | FPLC |
|---|
| Mode | SEC |
|---|---|
| Substances | Bovine Serum Albumin (BSA), 5-Carboxyfluorescein (5-FAM) |
| Key words | SEC, Desalting, Buffer exchange, group seperation, FPLC, Sepapure Desalting, AZURA FPLC |
| CAS number | 9048-46-8, 76823-03-5 |
for up to 10 mL/min
Article No.: SYS872112122
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for up to 10 mL/min
Article No.: SYS872101122
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