Pressure fluctuations caused by pump pulsation can present a challenge in FPLC, especially when working with pressure-sensitive columns. Here we analyzed the performance of the KNAUER PEEK pulse damper in combination with the AZURA® P 6.1L pump. The pulse damper was able to reduce pressure pulsation by up to 67% at small flow rates. The best working conditions for the pulse damper were determined at flow rates below 1ml/min in combination with a minimum back pressure of approximately 1.5 bar. At flow rates above 1ml/min, the dampening efficiency decreases significantly
Connection scheme of AZURA P 6.1L with pulse damper
Overlay of the external pressure signal at different flowrates, approx. 1.5 bar back pressure and water as eluent. Pressure signal without pulse damper (red), pressure signal with pulse damper (blue); 0.1 ml/min;
Overlay of the external pressure signal at different flowrates, approx. 1.5 bar back pressure and water as eluent. Pressure signal without pulse damper (red), pressure signal with pulse damper (blue); 0.5 ml/min
Overlay of the external pressure signal at different flowrates, approx. 1.5 bar back pressure and water as eluent. Pressure signal without pulse damper (red), pressure signal with pulse damper (blue); 1ml/min
Overlay of the external pressure signal at different flowrates, approx. 1.5 bar back pressure and water as eluent. Pressure signal without pulse damper (red), pressure signal with pulse damper (blue); 5 m
Comparison of the relative reduction of the pulsation for different flow rates and different back pressure (dark blue) ~1.5 bar; (red) ~4.5 bar (light blue) ~10 bar with water
| Method | FPLC |
|---|---|
| Mode | SEC |
| Substances | - |
| Key words | FPLC, SEC, pulse dsamper, P6.1L, biocomaptible |
| CAS number | - |
| Version | Version 1 / 10/2021 |
This pulse damper combines high damping performance with reliable, membrane-free assembly. Fully biocompatible, it can be easily integrated into all AZURA FPLC systems.
Article No.: AZZ10NB
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