This application note describes the use of the NanoScaler, KNAUER’s new Impingement Jets Mixing (IJM) benchtop system, to formulate LNPs of adequate size and polydispersity index (PdI) for various nucleic-acid payloads.
The authors performed measurements of Z-average size and polydispersity index for lipid nanoparticles, produced by IJM and microfluidic herringbone mixers to compare formulation reproducibility and efficiency. The comparison also includes transfection results.
Lipid nanoparticle illustration (cut-view)
Comparison of LNPs formulated with the NanoScaler and Herringbone micromixers: for pDNA-LNPs (A). NanoScaler showing slightly higher reproducibility
Comparison of LNPs formulated with the NanoScaler and Herringbone micromixers: for mRNA-LNPs. (B). NanoScaler showing slightly higher reproducibility
| Method | Lipid nanoparticle formulation (encapsulation) |
|---|---|
| Mode | Impingement jets mixing vs. microfluidic herringbone mixing |
| Substances | mRNA, pDNA |
| Key words | LNP, lipid nanoparticles, mRNA, pDNA, encapsulation efficiency, API, comparison, impingement jets mixing, herringbone microfluidic mixing, Z-average, PdI |
| Version | 2022 |
This application note describes the use of the NanoScaler, KNAUER’s new Impingement Jets Mixing (IJM) benchtop system, to formulate LNPs of adequate size and PdI. that enables the encapsulation of various nucleic-acid payloads with high reproducibility and efficiency. (8 pages)
This brochure provides a quick overview about the KNAUER Impingement Jets Mixing Skids – the technology, the process and the products, V7720US.
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