Biogenic amines are found in certain foods and play a role as regulatory agents in the human metabolism1. In food and beverages they are formed by enzymes out of raw materials or are generated by microbiological decarboxylation of amino acids. Many of them possess a strong pharmacologic effect and some are important as precursors of hormones and components of coenzymes. The biogenic amine poisoning leads to toxicological risks and health hazards that trigger psychoactive, vasoactive, and hypertensive effects resulting from the consumption of high amounts of biogenic amines in foods2.
Mixed amine standard,1 - derivatization peak, 2 - histamine, 3 - tyramine, 4 - putrescine, 5 - cadaverine
Chromatogram of derivatized blank (water). 1 - derivatization peak
Mix method setup for automated precolumn derivatization
Exemplarily tray positions for reagents A and B using the 84+3 autosampler tray
| Method | HPLC |
|---|---|
| Mode | RP |
| Substances | Histamine, tyramine, putrescine, cadaverine |
| Key words | Amines, biogenic amines, amino acids, histamine, tyramine, putrescine, cadaverine, precolumn derivatization, automated derivatization, OPA, ortho-phthaldialdehyd |
| CAS number | 51-45-6, 51-67-2, 110-60-1, 462-94-2 |
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