The use of the fungicide and food additive biphenyl has been severely restricted by the European Food Safety Authority (EFSA) since 2005. The multifunctional toxin is nevertheless found on numerous foodstuffs, which must therefore be strictly controlled. The RP HPLC method with simple UV detection shown here is a very fast and sensitive alternative to the previously used gas chromatography as a detection method.
Calibration curve of biphenyl in the range 0.01–10 μg/ml with 10 μl injection volume.
Chromatogram of biphenyl with a 10 μl injection of a standard solution of 0.01 μg/ml.
Method | HPLC UV |
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Mode | RP HPLC |
Substances | Biphenyl |
Key words | biphenyl, toxin, fungicide, food additive, EFSA, UV detection limit |
CAS number | 92-52-4 |
Version | Version 1/ 04/22 |
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