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Cyclodextrin purification Part 1

Application No.: VPH0066

Cyclodextrin purification Part 1


Method screening and overload studies

Cyclodextrins (CD) are macrocyclic compounds composed of five or more glycopyranosides. These ring structures can function as micro-capsules. An automated column screening was applied for method development to find best separation conditions of a CD mixture. Finally, an optimized method for CD purification was established.

  • Comparison of separation profiles from CD mixture and CD standards on C18 (dark blue) and C18H (red) columns (relevant time span of separation shown). CD mixture (30 mg/mL), CD8 (1mg/mL), CD 12,9,8 mixture (3mg/mL), CD10, 11 mixture (2 mg/mL); Y, Z uniden

  • Chromatograms of CD mixture mass overload studies; grey – 25 mg/ mL, green – 50 mg/mL, red – 75 mg/mL, dark blue – 100 mg/mL; C18 H 250 x 4 mm; 5 μm; 0.8 mL/min; 3 % methanol; 25°C; 50 μL

  • Chromatograms of CDmix volume overload studies. green – 50 μL, red – 75 μL, dark blue – 100 μL, light blue – 200 μL; C18H 250 x 4 mm; 5 μm; 0.8 mL/min; 3 % ,methanol; 25°C; 50 mg/mL

Technical Data

Application

Method HPLC

Separation conditions

Mode RP
Substances Cyclodextrine (CD), CD8, CD9, CD10, CD11, CD12
Key words Cyclodextrine mixture, preparative LC, RP, pharma, synthesis
CAS number -

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