The purification of natural products can be achieved by various techniques, including preparative single- and multi-column liquid chromatography. However, the development of efficient, fast and cost-effective methods for the purification of natural products from real samples remains a difficult and challenging task due to the complexity of the matrix. In contrast to conventional purification methods such as distillation, preparative chromatography uses less energy, consumes less water and chemicals while achieving higher yields [1]. In this application, a batch process for the purification of CBD (cannabidiol) from a CBD-rich cannabis extract was developed and optimized. Using the batch process, approximately 137 mg of CBD with a recovery of 95 % and a purity of 97 % was obtained from a 4 ml extract.
System setup of preparative HPLC system
Overlay chromatograms of three preparative measurements with premixed eluents
Overlay chromatogram of preparative overload tests
Chromatogram section of preparative fractionation over CBD peak
| Method | Preperative HPLC |
|---|---|
| Mode | RP |
| Substances | Cannabidiol (CBD), Cannabichromene (CBC), Tetrahydrocannabinol (THC) |
| Key words | HPLC, preparative chromatography, RP, purification of natural products, method development, scale up, CBD, CBC, THC |
| CAS number | 13956-29-1, 20675-51-8, 1972-08-3 |
| Version | Version 01 / 11.07.2024 |
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