Solid phase extraction (SPE) is a useful technique to enhance detection limits to analyse even very small amounts of compounds. Most often SPE is carried out manually but coupled to an HPLC system, the so called online SPE technique allows an automated sample analysis. The used system configuration makes it possible to switch between direct injection via autosampler and injection with an auxiliary pump (aux/feed pump) without changing/replumbing the system.
Exemplary structures of selected PAHs
Exemplary chromatogram of 16 PAH according EPA method 610 [2], red – fluorescence trace, blue – UV trace 230 nm
Used valves in system configuration
Manual SPE load; multi-injection valve: Pos 1, 8-port 2-position: Pos 1
SPE elution; multi-injection valve: Pos 1, 8-port 2-position: Pos 2
SPE load via autosampler; multi-injection valve: Pos 3, 8-port 2-position: Pos 1
SPE load via aux pump; multi-injection valve: Pos 4, 8-port 2-position: Pos 1
SPE elution; multi-injection valve: Pos 4, 8-port 2-position: Pos 2
| Method | HPLC |
|---|---|
| Mode | RP |
| Substances | EPA 610 PAH mixture, fluoranthene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, benzo[g,h,i]perylene, indeno[1,2,3-cd]pyrene |
| Key words | HPLC, online SPE, RP, PAH, polycyclic aromatic hydrocarbons, solid phase extraction, multi-injection valve, 8-port 2-position valve, EPA 610, automation, sample preparation, fluorescence |
| CAS number | 206-44-0, 205-99-2, 207-08-9, 50-32-8, 191-24-2, 193-39-5 |
| Version | Version 1/ 11/2022 |
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