Vitamins are essential micronutrients that are needed in small amounts for various roles throughout the human body. Vitamins are divided into two groups: water-soluble (B-complex vitamins and C vitamins) and fat-soluble vitamins (A, D, E, and K). The fat-soluble vitamins are stored in the body for long periods of time and generally pose a greater risk for toxicity when consumed in excess than water-soluble vitamins [1]. Here, an analytical HPLC method based on size exclusion chromatography is described.
Chromatogram of a mixed standard of fat-soluble vitamins at 0.05 mg/mL (A, D, K) and 0.09 mg/mL (E), 1) vitamin A palmitate, 2) vitamin E, 3) vitamin D3, 4) vitamin K
Calibration curves for vitamin D3 (red), vitamin A (green), vitamin K (blue), and vitamin E (grey); corresponding linearity values are indicated
| Method | HPLC |
|---|---|
| Mode | GPC/SEC |
| Substances | vitamin A palmitate, vitamin E, vitamin D3, vitamin K |
| Key words | GPC, SEC, size exclusion, vitamins, fat soluble vitamins, vitamin A palmitate, vitamin E, vitamin D3, vitamin K |
| CAS number | 79-81-2, 67-97-0, 10191-41-0, 58-27-5 |
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