Vitamins are divided into two different groups: water-soluble and fat-soluble. The fat-soluble vitamins are A, D, E and K – the water-soluble vitamins are vitamin C and the vitamin B complex. Based on their different chemical properties, they are usually analyzed using different methods and modes. Reversed phase HPLC is very common for the water-soluble vitamins whereas normal phase mode is often applied for the fat-soluble vitamins. Nevertheless, it is possible that the analytes can occur in the same matrix. For this reason, a simultaneous determination of both water- and fat-soluble vitamins was developed in this work, resulting in a reduction in solvent consumption and total analysis time.
Chromatogram of mixed standard; (1) vitamin B2; (2) vitamin B9; (3) vitamin B12; (4) vitamin A palmitate; (5) vitamin D3; (6) vitamin E, UV detection with wavelength switching
Calibration curves
| Method | HPLC |
|---|---|
| Mode | RP |
| Substances | Vitamin B12, vitamin B2, vitamin B9, vitamin E, vitamin D3, vitamin A palmitate |
| Key words | HPLC, RP, vitamins, water-soluble vitamins, fat-soluble vitamins, Vitamin B12, vitamin B2, vitamin B9, vitamin E, vitamin D3, vitamin A, riboflavin, folic acid, cyanocobalamin |
| CAS number | 68-19-9, 83-88-5, 59-30-3, 10191-41-0, 67-97-0, 79-81-2 |
| Version | Version 1/ 10/2021 |
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