Publications

The following selected scientific publications have been made by KNAUER scientists, in cooperation with KNAUER or using KNAUER HPLC systems or columns. Some of the publications may only be available on purchase.

The publications are listed in original language. 

2024

"Sustainable cannabinoids purification through twin-column recyclingchromatography and green solvents"

In the present study, twin-column recycling chromatography has been employed for the purification of a Cannabis extract by using a green solvent, ethanol, as the mobile phase. In particular, the complete removal of the psychoactive tetrahydrocannabinol (THC) from a Cannabis extract rich in cannabidiol (CBD) was achieved under continuous conditions.

By: Greta Compagnin, Chiara De Luca, Chiara Nosengo, Martina Catani, Alberto Cavazzini, Giorgia Greco, Yannick Krauke, Simona Felletti

In: Springer Nature - Anal Bioanal Chem (2024). Published: 15 May 2024. Open Access https://doi.org/10.1007/s00216-024-05332-7

_{Keywords: Recycling chromatography, Cannabinoids, Cannabis sativa L., THC depletion, Green solvent}
 

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2023

"Green cannabigerol purification through simulated moving bed chromatography"

In this work, CBG has been purified from a real cannabis extract by means of simulated moving bed chromatography. The proposed application is very promising, allowing to achieve a CBG extract free of tetrahydrocannabinol (a psychoactive cannabinoid) with 100% recovery and 97% final purity by using a faster and greener method if compared to traditionally used ones.

By: Chiara De Luca, Yannick Krauke, Svea Stephan, Giorgia Greco, Greta Compagnin, Alessandro Buratti, Alberto Cavazzini, Martina Catani, Simona FellettiGiorgia Greco · Yannick Krauke · Simona Felletti

In: Elsevier - Green Analytical Chemistry, Volume 6, September 2023, 100066, https://doi.org/10.1016/j.greeac.2023.100066

_{Keywords: Cannabinoids, Cannabis Sativa L., Simulated moving bed. Green continuous purification, Modeling}
 

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"Purification and Isolation of Cannabinoids: Current Challenges and Perspectives"

This review reports on the challenges and future perspectives in the purification and isolation of major and minor cannabinoids from cannabis extracts.

By: Simona Felletti, Greta Compagnin, Yannick Krauke, Svea Stephan, Giorgia Greco, Alessandro Buratti, Tatiana Chenet, Chiara De Luca, Martina Catani, Alberto Cavazzini

In: LCGC Europe, Vol 36, Issue 04, pp 122-131, April 2023, https://doi.org/10.56530/lcgc.eu.jp5571c5.

_{Keywords: Cannabinoids; Cannabis sativa L.; isolation, purification, extracts, challenges}
 

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2022

"Magnetic/flow controlled continuous size fractionation of magnetic nanoparticles using simulated moving bed chromatography"

Continuous size fractionation of magnetic particles with diameters between 80 and 1000 nm. Separation sharpness of 0.752. Yield of 99.9%. High space-time yield of 2.94 g/(L*h). KNAUER SMB systems were used for this research.

By: Laura Kuger, Carsten-René Arlt, Matthias Franzreb

In: Talanta, Volume 240, 1 April 2022, 123160.

The complete article can be found at: https://doi.org/10.1016/j.talanta.2021.123160

_{Keywords: Fractionation; magentic particles; high yield; simulated moving bed chromatography}
 

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"Investigating the effect of polarity of stationary and mobile phases on retention of cannabinoids in normal phase liquid chromatography"

By: Chiara De Luca, Alessandro Buratti, Yannick Krauke, Svea Stephan, Kate Monks, Virginia Brighenti, Federica Pellati, Alberto Cavazzini, Martina Catani, Simona Felletti

In: Anal Bioanal Chem 2022 Feb 10. doi: 10.1007/s00216-021-03862-y.

The complete article can be found at: https://pubmed.ncbi.nlm.nih.gov/35146546/

_{Keywords: Cannabinoids; Cannabis sativa L.; HPLC; Hemp; Normal phase; Polar-bonded phases}
 

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2021

"Continuous fractionation of nanoparticles based on their magnetic properties applying simulated moving bed chromatography"

Classification of nanoparticles with similar size by using magnetic chromatography. Transfer of the process to a simulated moving bed chromatography. Contaminant-depletion up to 100%. KNAUER SMB systems were used for this research.

By: Carsten-Rene Arlt , Dominik Brekel , Matthias Franzreb

In: .Separation and Purification Technology 259 (2021) 118123

The complete article can be found at: https://doi.org/10.1016/j.seppur.2020.118123

_{Keywords: Nanoparticles; classification; magnetic chromatography; simulated moving bed; high purity}
 

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2019

"Analyse von Holzzuckern und Uronsäuren"

By Kristin Folmert, Jan Wendrich, Kate Monks

In: GIT Labor-Fachzeitschrift 7/2019, S. 32–34, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

The complete article can be found at: https://www.git-labor.de/forschung/life-sciences-biotechnologie/analyse-von-holzzuckern-und-uronsaeuren

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"Large‐Ring Cyclodextrins as Chiral Selectors for Enantiomeric Pharmaceuticals"

Christian Sonnendecker,  Dr. Sebastian Thürmann,  Dr. Cédric Przybylski,  Franziska D. Zitzmann,  Nicole Heinke,  Dr. Yannick Krauke,  Dr. Kate Monks,  Prof. Dr. Andrea A. Robitzki,  Prof. Dr. Detlev Belder,  Prof. Dr. Wolfgang Zimmermann

First published: 18 March 2019
In: Angewandte Chemie, a Journal of the Gesellschaft Deutscher Chemiker

The complete article can be found at: https://doi.org/10.1002/anie.201900911
 

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"Von Zucker und Wasser"

Grüne Zuckeranalytik für Jedermann // Beitrag aus der LABORPRAXIS 3-2019
Die Analytik von Zuckern reicht von der einfachen Bestimmung per Farbreaktion bis hin zur komplexen Quantifizierung mittels Massenspektrometrie. Wie die Zuckeranalytik per Hochleistungs-Flüssigkeitschromatographie (HPLC) gleichermaßen einfach wie „grün“ möglich ist, beschreibt diese Applikation.

Lesen Sie hier den ganzen Artikel.
 

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2018

"Fractionation of Soda Pulp Lignin in Aqueous Solvent through Membrane-Assisted Ultrafiltration"

By Chiara Allegretti†, Simon Fontanay‡, Yannick Krauke§, Matthias Luebbert§, Alberto Strini∥, Julien Troquet‡, Stefano Turri† , Gianmarco Griffini*† , and Paola D’Arrigo*†⊥# 

In: ACS Sustainable Chem. Eng., Article ASAP

The complete article can be found at: https://pubs.acs.org/doi/pdf/10.1021/acssuschemeng.8b01410

_{Keywords: Biomass valorization, Lignin, Ultrafiltration, Membranes, Fractionation}

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"Continuous Selective Hydrogenation of Refametinib Iodo-nitroaniline Key Intermediate DIM-NA over Raney Cobalt Catalyst at kg/day Scale with Online UV–Visible Conversion Control"

By: Mourad Ben Said†, Todor Baramov‡§, Tanja Herrmann†, Michael Gottfried†, Jorma Hassfeld‡, and Stefan Roggan*‡
    † Engineering & Technology, Bayer AG, D-51368 Leverkusen, Germany
    ‡ Pharmaceuticals Division, Bayer AG, D-42096 Wuppertal, Germany
    § Leibniz-Institut für Katalyse e.V. (LIKAT), Albert-Einstein-Str. 29a, D-18059 Rostock, Germany

In: Org. Process Res. Dev., 2017, 21 (5), pp 705–714

The complete article can be found at: https://pubs.acs.org/doi/10.1021/acs.oprd.7b00039

_{Keywords: flow chemistry, continuous synthesis, pharma intermediate, dosing pump, metering, feed, flow-through, UV detection, remote flow cell}
 

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"Antikörperreinigung mit Glasmonolithen"

By Franziska Scherz, Ulrike Krop, Kate Monks, Michael G. Weller

In: GIT Labor-Fachzeitschrift 3/2018, S. 24–25, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

The complete article can be found at: http://www.git-labor.de/forschung/materialien/antikoerperreinigung-mit-glasmonolithen

_{Keywords: FPLC, kamelide Antikörper, schwere-Ketten-Antikörper}
 

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2017

"Peptidliganden zur Aufreinigung kamelider Schwere-Ketten-Antikörper"

By: Matthias Paschke and Veit Althoff

In: Biospektrum 4/2017, page 424

The complete article can be found at: https://link.springer.com/article/10.1007/s12268-017-0821-5

_{Keywords: FPLC, kamelide Antikörper, schwere-Ketten-Antikörper}
 

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“Determination of Aflatoxin M1 in Milk”

By Juliane Böttcher and Kate Monks

In: The Analytical Scientist 51, April 2017, page 49

The complete article can be found at: https://theanalyticalscientist.com/issues/0417/determination-of-aflatoxin-m1-in-milk/

_{Keywords: Aflatoxins, mycotoxins, milk analysis, post column derivatisation,, solid phase extraction, SPE} 
 

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"The Infinite Column"

By Christian Benkhäuser, Hagen Schlicke
In: The Analytical Scientist

The complete article can be found at: https://theanalyticalscientist.com/app-notes/0017/the-infinite-column/ 

_{Keywords: preparative HPLC. Peak Recycling, Vitamin E, Tocopherol}
 

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2016

“High-performance liquid chromatography with electrospray ionization ion mobility spectrometry: Characterization, data management, and applications”

By: Martin Zühlke, Daniel Riebe, Toralf Beitz, Hans-Gerd Löhmannsröben, Sandro Andreotti, Knut Reinert, Karl Zenichowski, Marc Diener

In: J. Sep. Sci. 2016, 39, 4756–4764

The complete article can be found at: http://onlinelibrary.wiley.com/doi/10.1002/jssc.201600749/pdf

_{Keywords: Amino acids / Electrospray ionization / Ion mobility spectrometry /Pesticides / Two-dimensional separations}
 

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"Sensitive Online SPE Determination of Bisphenol A in Water Samples"

By Juliane Böttcher and  Mareike Margraf 
In: The Analytical Scientist 44, September 2016, page 49

The complete article can be found at: https://theanalyticalscientist.com/fileadmin/tas/pdf-versions/TAS_Issue_0916.pdf#page=49

_{Keywords: bisphenol A, BPA, drinking water analysis, solid-phase extraction, SPE}
 

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"FPLC versus Analytical HPLC: Two Methods, One Origin, Many Differences"

By: Stephanie Runde
In: The Column, Volume 12, Issue 15, August 2016, page 12–16

The complete article can be found at: http://www.chromatographyonline.com/fplc-versus-analytical-hplc-two-methods-one-origin-many-differences-0

_{Keywords: FPLC, Bio LC, HPLC, biomolecules, size-exclusion, ion-exchange, affinity, IMAC}
 

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"Are the Lights on Green? - Micro-LC’s Road to Routine Analysis"

By: Thorsten Teutenberg, Terence Hetzel, Denise Loeker, Juri Leonhardt, Philipp Schulze.
In: GIT Laboratory Journal, June 14 2016

The complete article can be found at: http://www.laboratory-journal.com/science/chemistry-physics/are-lights-green

_{Keywords: nano LC, Micro LC, HPLC, fibre optics, extra column volume}
 

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"Characterization of commercially available peptidases in respect of the production of protein hydrolysates with defined compositions using a three-step methodology"

By: Michael Merz, Wolfgang Claaßen, Daniel Appel, Pieter Berends, Swen Rabe, Imre Blank, Timo Stressler, and Lutz Fischer.
In: Journal of Molecular Catalysis B: Enzymatic 127, May 2016, pages 1-10

The complete article can be purchased at: https://doi.org/10.1016/j.molcatb.2016.02.002 

_{Keywords: Commercial peptidases, peptidase composition, amino acid release; automated enzymatic hydrolysis; functional hydrolysates}
 

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2015

"Sensitive Analysis of the Lactose Content of Lactose-Free Labelled Products Using HPAEC-PAD"

By: Mareike Margraf and Dr. Kate Monks
In: LCGC Europe, The Applications Book, December 2015, page 680 f.

http://images2.advanstar.com/PixelMags/lcgc-eu/digitaledition/12-2015.html

_{Keywords: food, lactose, lactose-free}
 

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"Determination of Lactose in Lactose-free Products Using HPAEC-PAD",

By: Mareike Margraf and Dr. Kate Monks
In: The Analytical Scientist, October 2015, page 57

https://theanalyticalscientist.com/fileadmin/tas/pdf-versions/TAS_Issue_1015.pdf#57

_{Keywords: High performance anion-exchange chromatography, pulsed amperometric detection, lactose intolerance, lactose thresholds, lactase enzyme, glucose, galactose, sugar content, dairy food analysis, electrochemical detection, trace analysis of carbohydrates}
 

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"An electrospray ionization-ion mobility spectrometer as detector for high-performance liquid chromatography"

By: Martin Zühlke, Daniel Riebe, Toralf Beitz, Hans-Gerd Löhmannsröben, Karl Zenichowski, Marc Diener, and Michael W. Linscheid.
In: European Journal of Mass Spectrometry. Volume 21 Issue 3 (2015), Special Issue: Celebrating the 20th Anniversary of EJMS, pages 391–402

http://www.impublications.com/content/ejms-table-contents?issue=21_3

_{Keywords: multijet electrospray, electrospray mechanism, source design, imaging, fast multidimensional chromatography, full HPLC flowrate compatibility, split-free electrospray source, ion-mobility separation, collision cross section measurement, separation of isomers, analysis of pesticides, neuroleptics and antibiotics}
 

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"Comparison of Compounds in Bourbon Vanilla Extract and Vanilla Flavour"

By: Mareike Margraf 
In: The Application Notebook, LC/Gc, Jul 01, 2015

http://www.chromatographyonline.com/comparison-compounds-bourbon-vanilla-extract-and-vanilla-flavour

^{Keywords: food, 4-hydroxybenzoic acid, vanillic acid, 4-hydroxybenzaldehyde, vanillin, natural Bourbon vanilla flavor, guaiacol, ethylvanillin, coumarin, eugenol, synthetic vanilla flavor, vanillin synthesis process, artificial vanilla flavour}
 

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"Automated solid-phase extraction coupled online with HPLC-FLD for the quantification of zearalenone in edible oil"

By: Sarah S. Drzymala, Stefan Weiz , Julia Heinze, Silvia Marten, Carsten Prinz, Annett Zimathies, Leif-Alexander Garbe, Matthias Koch.
In: Analytical and Bioanalytical Chemistry, May 2015, Volume 407, Issue 12, pages 3489-3497 

http://link.springer.com/article/10.1007%2Fs00216-015-8541-5

_{Keywords: dynamic covalent hydrazine chemistry (DCHC), maize germ oil, mycotoxin}
 

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"Innovative Chromatography System Revolutionizes Purification and Yield"

By: S. Schagen, M. Fuchs, U. Maschke, S. Marten, A. Knauer, M. Bavand, and T. Müller-Späth 
In: Global Ingredients & Formulations Guide 2015; Chapter VI - Production Technologies, pp. 156 – 162

http://www.shopsofw.com/Englische-Buecher/Global-Ingredients---Formulations-Guide/Global-Ingredients-Formulations-Guide-2015.html?XTCsid=g5mhstf5lu1s08cdjm8m1h8g66

^{Keywords: multi-column chromatography, Continous Chromatography, MCSGP, FPLC, Capture SMB, Affinity Chromatography, Protein A column, mAb purification}