CBD purification – Part 2: Transfer and optimization of a purification method in preparative scale

The purification of natural products can be achieved by various techniques, including preparative single- and multi-column liquid chromatography. However, the development of efficient, fast and cost-effective methods for the purification of natural products from real samples remains a difficult and challenging task due to the complexity of the matrix. In contrast to conventional purification methods such as distillation, preparative chromatography uses less energy, consumes less water and chemicals while achieving higher yields [1]. In this application, a batch process for the purification of CBD (cannabidiol) from a CBD-rich cannabis extract was developed and optimized. Using the batch process, approximately 137 mg of CBD with a recovery of 95 % and a purity of 97 % was obtained from a 4 ml extract.

Methode	Preperative HPLC
Modus	RP
Substanzen	Cannabidiol (CBD), Cannabichromene (CBC), Tetrahydrocannabinol (THC)
Schlüsselwörter	HPLC, preparative chromatography, RP, purification of natural products, method development, scale up, CBD, CBC, THC
CAS Nummer	13956-29-1, 20675-51-8, 1972-08-3
Version	Version 01 / 11.07.2024