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When they go LOW, we go HIGH - Comparing sensitivity levels for the analysis of fluorescence-labeled proteins

Applikationsnr.: VTN0005

When they go LOW, we go HIGH - Comparing sensitivity levels for the analysis of fluorescence-labeled proteins

The use of a fluorescence detector increases the sensitivity of the chromatographic analysis. In this Tech Note we evaluated the different sensitivity modes of the fluorescence detector with a fluorescein-labeled protein and compared it to the UV signal.

  • Fig. 1 Overlay of FITC-BSA fluorescence peak measured with different sensitivity modes (red line HIGH, green line MED, blue line LOW). 1.3 μg of sample 2 was injected.

  • Fig. 2A Overlay of FITC-BSA fluorescence (red) and UV 280 nm (blue) peak measured with HIGH sensitivity. 1.3 μg of sample 2 was injected.

  • Fig. 2B Overlay of FITC-BSA fluorescence (red) and UV 280 nm (blue) peak measured with MED sensitivity. 1.3 μg of sample 2 was injected.

  • Fig. 2C Overlay of FITC-BSA fluorescence (red) and UV 280 nm (blue) peak measured with LOW sensitivity. 1.3 μg of sample 2 was injected.

  • System configuration

Technische Daten

Applikation

Methode FPLC

Trennbedingungen

Modus SEC
Substanzen Fluorescein isothiocyanate isomer I (FITC), Bovine Serum Albumin (BSA)
Schlüsselwörter SEC, FPLC, AZURA FPLC, fluorescence, BSA, FITC, fluorescein-labeled protein
CAS Nummer 3326-32-7, 9048-46-8

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