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Systematic and efficient method scale-up for peptide purification

Applikationsnr.: VBS0084

Systematic and efficient method scale-up for peptide purification

Peptides, crucial in therapeutic advancements, are synthesized through refined methods in biotechnology. High-performance liquid chromatography (HPLC) plays a pivotal role, ensuring the purity of synthesized peptides. Linear scale-up of HPLC methods becomes imperative for enhancing productivity and method robustness in pharmaceutical and analytical laboratories. This process involves meticulous adjustments to parameters, such as column size and solvent composition, to maintain consistent chromatographic performance. Scaling up begins with optimizing the method on a larger scale, ensuring minimal sample loss while maximizing productivity.

  • Analytical overview gradient; (1) Angiotensin I

  • Focused gradient and wash step

  • Top to bottom: 100, 50, 25, 10, 5 µl injection volume

  • Preparative run (zoomed); 2000 µl injection volume; 2.5 ml fractions

Technische Daten

Spezifikationen

Methode Preparative HPLC
Modus RP
Substanzen Angiotensin I
Schlüsselwörter HPLC, preparative, RP, purification, Angiotensin, Angiotensin I, TFA, Trifluoroacetic acid, scale-up, overload, UV, hormone, Peptides, Biotechnology, High-performance liquid chromatography (
CAS Nummer 484-42-4; 9041-90-1
Version Version 1 / 07/2024

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