New high-pressure valve to switch between four columns for easy HPLC method development

When developing an HPLC method, one of the initial steps involves screening a variety of columns to determine which offers the most effective separation of the analytes. While this can be performed manually, an automated approach is generally preferable, as it enables rapid switching between multiple columns within a short timeframe. Automated valve systems present a viable solution for this purpose. Two common configurations are employed: (1) using two multiposition valves with columns arranged between them, or (2) employing a specialized column switching valve designed to accommodate all columns simultaneously. Here, we introduce a new high-pressure column switching valve capable of connecting up to four columns, in addition to providing a bypass path. To demonstrate its utility, the separation profiles of a standard mixture were evaluated on four different columns under two distinct solvent gradient conditions. This exemplary screening illustrates the advantages of employing a single column switching valve for method development and other applications that require efficient and reliable switching between multiple columns.

Methode

HPLC

Modus

Substanzen

Vanillic acid, syringic acid, p-coumaric acid, ferulic acid, benzoic acid, quercetin, kaempferol

Schlüsselwörter

Column selection valve, method development, solvent screening, stationary phase screening, automatization

CAS Nummer

121-34-6, 530-57-4, 501-98-4, 1135-24-6, 65-85-0, 117-39-5, 520-18-3

Version

Version1 / 12/2024