Large scale purification – of oligonucleotides with ion exchange chromatography

The field of oligonucleotide therapeutics has made remarkable progress in recent years leading to a growing need for purified oligonucleotides. One of the classic oligonucleotide production techniques is chemical synthesis followed by a chromatographic purification step. Especially for up scaling, ion exchange chromatography is the ideal method due to its high loading capacity combined with high resolution and yield. Here we present the work of BianoGMP GmbH. The company specialised in the production of high purity and quality oligonucleotides in quantities of up to several grams for research and development and clinical programmes. BianoGMP GmbH has many years of experience in the development of therapeutic oligonucleotides with a focus on GMP services and manufacturing process optimisation. Here we demonstrate a successful large-scale ion exchange chromatography purification of an oligo dT with 95% purity and 90% yield.