Two-step purification is a special multicolumn chromatography solution. Two independent methods, each with their associated specific column, are used to realize the purification of the target molecule without manual interference. The principle here is that the protein sample is applied on the first column. During elution of the protein, the protein peak is detected triggering the collection of the eluted protein in a storage loop or storage vessel/container. The protein is then automatically applied on the second column to further enhance the quality and or purity of the purified protein. Several system set ups can be used to automate the purification. In this first section of the three-part guide the principles of two-step purification are discussed.
| Methode | FPLC |
|---|---|
| Schlüsselwörter | Two step purification, purification, protein purification, biochromatography, guide, principles |
| Version | Version 1 / 12/2020 |
VTN0013
für bis zu 50 ml/min
Artikelnummer: SYS862113122
Zum Produkt
Speziell für FPLC-Kunden: biokompatibles Multifunktions-Injektionsventil (PEEK, 1/16")
Artikelnummer: AVN94CE
Zum Produkt
Ventil für die Auswahl von 5 Säulen, Flussumkehr und Bypass
Artikelnummer: AVZ52CE
Zum Produkt
Multipositions Ventil, 8 Port, PEEK, PEEK, 1/16'', 240 bar, 0.75 mm Bohrgröße
Artikelnummer: AVS34CE
Zum Produkt
VU 4.1 Ventilantrieb für V 4.1 Ventile
Artikelnummer: AWA01XA
Zum Produkt
Assistentenmodul P 4.1S, 50 bar, 50 ml, Keramik
Artikelnummer: APG12FB
Zum Produkt
Assistentenmodul P 4.1S, 50 bar, 10 ml, Keramik
Artikelnummer: APG12EB
Zum Produkt
Assistentenmodul VU 4.1 für Ventile V 4.1
Artikelnummer: AWA04
Zum Produkt
für bis zu 50 ml/min
Artikelnummer: SYS875413122
Zum ProduktWir helfen Ihnen gerne!
