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Determination of sugars in honey - comparison of refractive index and light scattering detection

Applikationsnr.: VFD0169

Determination of sugars in honey - comparison of refractive index and light scattering detection

Determination of the sugar content and their composition in honey allows a prediction about the origin of honey and determines how it should be labeled. Because of honey being one of the most popular natural food stuffs and its growing consumption, it is necessary to carry out quality control. The dedicated AZURA® Sugar Analytical System with autosampler upgrade is suitable for this application.

  • ELSD trace (red) and RID trace (blue) of a mixed standard at 0.60 mg/mL; 1) sucrose, 2) maltose, 3) glucose, 4) fructose

  • Overlay of measured samples; ELSD traces: Knauer honey – blue, blossom honey – red, agave nectar – green; 1) sucrose, 2) maltose, 3) glucose, 4) fructose

  • Overlay of measured samples; RID traces: Knauer honey – blue, blossom honey – red, agave nectar – green; 1) sucrose, 2) maltose, 3) glucose, 4) fructose

Technische Daten

Spezifikationen

Methode HPLC
Modus Ion exclusion, Ligand exchange
Substanzen Glucose, sucrose, maltose, fructose
Schlüsselwörter Honey, sugar, Eurokat, RID 2.1L, ELSD, light scattering detector, glucose, fructose, maltose, sucrose, AOAC
CAS Nummer 57-48-7, 14431-43-7, 57-50-1, 6363-53-7

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