Ion exchangers are used in a variety of protein purification protocols. This application compares equivalent columns, a weak and a strong anion exchanger as well as a weak and a strong cation exchanger from two different vendors.
The columns were comparable in all assessed cases.
Chromatograms of the separation of Ribonuclease A (1), Cytochrome C (2), and Lysozyme (3) with weak cation exchange chromatography columns
Chromatograms of the separation of Ribonuclease A (1), Cytochrome C (2), and Lysozyme (3) with strong cation exchange chromatography columns
Chromatograms of the separation of Conalbumin (1), α-Lactoglobin (2), and soy bean Trypsin inhibitor (3) with weak anion exchange chromatography columns
Chromatograms of the separation of Conalbumin (1), α-Lactoglobin (2) and soy bean Trypsin inhibitor (3) with strong anion exchange chromatography columns
| Methode | FPLC |
|---|
| Modus | IEC |
|---|---|
| Substanzen | AIEC: Conalbumin, a-Lactalbumin, soy bean trypsin inhibitor; CIEC: Cytochrome C, Lysozyme, Ribonuclease A |
| Schlüsselwörter | FPLC, IEC, CIEC, AIEC, AZURA FPLC, Sepapure SP, Sepapure CM, Sepapure Q, Sepapure DEAE |
| CAS Nummer | 1391-06-6, 9051-29-0, 9035-81-8, 9007-43-6,12650-88-3, 9001-99-4 |
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