CBD purification – Part 1: Preparative HPLC method development in analytical scale and scale-up

The purification of compounds from natural sources can be achieved by various techniques, including single- and multi-column liquid chromatography. However, the development of efficient, fast and cost-effective methods for the purification of natural products from i.e. extracts can be a difficult and challenging task due to the complexity of the matrix. In contrast to conventional purification methods such as distillation, preparative chromatography uses less energy, consumes less water and chemicals while achieving higher yields [1]. In this application, a batch process for the purification of CBD (cannabidiol) from a CBD-rich cannabis extract was established. The application is devided into two Application Notes, first, an analytical method was developed and optimized. Second, CBD was purified using the scaled-up method on a preparative system.

Methode	Preparative HPLC
Modus	RP
Substanzen	Cannabidiol (CBD), Cannabichromene (CBC), Tetrahydrocannabinol (THC)
Schlüsselwörter	HPLC, preparative chromatography, RP, purification of natural products, method development, scale up, CBD, CBC, THC
CAS Nummer	13956-29-1, 20675-51-8, 1972-08-3
Version	Version 01/11.07.2024