Automated two step purification with cation exchange chromatography and desalting

In many cases protein purification requires two or even more steps to achieve sufficient purity. The transition from one step to another can be very time consuming and requests manual interaction. These limitations illustrate the need for FPLC systems enabling automated two step purification. This application highlights the possibilityof combining two subsequent chromatography protocols. This was achieved using the KNAUER Lab standard Multi Method FPLC system adapted for automatic two step purification. An exemplary separation of model proteins by cation exchange chromatography followed by desalting is shown. This approach can easily be adapted to various protein purification protocols.

Methode	Two step purification
Modus	IEC
Substanzen	Cytochrome C, Ribonuklease A
Schlüsselwörter	FPLC, IEC, protein purification, Cytochrome C, Ribonuklease A, AZURA® Bio Lab system
CAS Nummer	Cytochrome C: CAS 9007-43-6; Ribonuklease A: CAS 9001-99-4
Version	Version 1 / 12/2020