The mass and volume loadabilities always depend on the specific sample and application as well as on the stationary phase filled in the HPLC column. The following chart only gives a first clue and can be higher or lower than the given numbers. Scale-up factor (SF) calculation: SF = ID²(preparative)/ID²(analytical)
- Example: Scale-up from 250 x 4 mm ID column to 250 x 20 mm ID column: SF = ID²(preparative)/ID²(analytical) = 20²/4² = 400/16 = 25
