Example of a typical two step purification system
Several FPLC system set ups can be used to automate the purification. Some of the most common set ups are the basic set up and the set up with a system pump. We assume that the biocompatible multi-injection valve (AVN94CE) is included within the FPLC system. For all set ups a column selection valve (AVZ52CE) to select the different columns and an outlet valve (AVS34CE) to redirect the flow and collect the first peak must be installed in the system.