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Start up, shut down - Screening of pressure sensitive columns with the 8 port 2-position valve

Applikationsnr.: VTN0008

Start up, shut down - Screening of pressure sensitive columns with the 8 port 2-position valve

Using valves in FPLC is most often necessary due to manual injection, column switching or fractionation. But also common analytical HPLC applications can be improved by adding valves. Widespread analytical application areas are for example column screenings to find the suitable column. A large variety of valves is available, but not all are suitable for the screening of pressure sensitive columns. This note describes an application of a KNAUER 8 port 2-position valve in a screening process with Eurokat columns.

  • Switching of 8 port 2-position valve. Position 1 – left, position 2 - right

  • Mixed standard on Eurokat Na. 1 - Cellobiose, 2 - Mannitol/Glucose, 3 - Xylose, 4 - Xylitol, 5 - Glycerin/Arabinose

  • Mixed standard on Eurokat Ca. 1 - Cellobiose, 2 - Glucose, 3 - Xylose, 4 - Arabinose, 5 - Glycerin, 6 - Mannitol, 7 - Xylitol

Technische Daten

Spezifikationen

Methode HPLC
Modus Ion exclusion, Ligand exchange
Substanzen Cellobiose, mannitol, glucose, xylose, xylitol, glycerin, arabinose
Schlüsselwörter Eurokat, carbohydrates, column screening, sugar, PSDVB, polymer column, degree of polymerization, RID, refractive index
CAS Nummer 528-50-7, 87-78-5, 58367-01-4, 609-06-3, 87-99-0 , 56-81-5 , 147-81-9
Version 1/10-2020

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