EN DE

What is two step purification?

KNAUER Multi Method FPLC System, Lab standard

In the basic set up a Lab standard KNAUER Multi Method FPLC System for all Bio-Chromatography methods is adapted. Next to the column selection valve and the outlet valve no other components must be added to the system. The reinjection position of the outlet valve is connected to the sample pump inlet of the injection valve, so that the syringe port is still accessible for sample injection. The sample is injected via the sample loop of the injection valve. The first peak is collected in the sample loop of the injection valve as well and reinjected onto the second column. 

Consideration: This set up is limited to small sample volumes. The volume of the elution peak of the first column should be equal or even smaller than the injection volume due to diffusion effects that will result in sample loss. 

To overcome the problem of the small sample volume a variation of this set up is to inject the sample via the major pump. For this set up some additional changes must be implemented. The filter cartridge of the pressure senor must be replaced by a dummy and we strongly advise to install an inline filter in front of the column selection valve. 

Wir sind persönlich für Sie da –
in ganz Deutschland, Österreich und der Schweiz!

Haben Sie Fragen zu unseren Produkten oder benötigen Sie ein Angebot?
Kontaktieren Sie uns – wir helfen Ihnen gerne weiter!

Braunschweig

Dr. Diana Rohrbeck
 

Tel: +49 151 15943077
Meeting vereinbaren

E-Mail schreiben

Berlin

Oskar Klak, M.Sc.
 

Tel: +49 170 2862155
Meeting vereinbaren

E-Mail schreiben

Frechen

Sandra Lehmann, M.Sc.
 

Tel: +49 171 4113554
Meeting vereinbaren

E-Mail schreiben

Leipzig

Dipl.-Ing. (FH) Micha Hinderer
 

Tel: +49 151 43536226
Meeting vereinbaren

E-Mail schreiben

Frankfurt am Main

Can Neis, BE
 

Tel: +49 171 1227074
Meeting vereinbaren

E-Mail schreiben

München

Dipl.-Ing. (FH) Christian Ulbricht
 

Tel: +49 170 9275967
Meeting vereinbaren

E-Mail schreiben

Österreich & Schweiz

Marius Segl, M.Sc.
 

Tel: +43 676 3806550
Meeting vereinbaren

E-Mail schreiben

Finden Sie Ihre/n Ansprechpartner/in
in Deutschland ...

... nach Postleitzahl

Brauchen Sie mehr Informationen?

Kontaktieren Sie uns, wir helfen Ihnen gerne!

+49 30 809727-0

Mo – Fr
07:00 – 17:00 CE(S)T

sales@knauer.net

24 / 7
 

Besuchen Sie uns auf: